Displacement chromatography on synthetic ion-exchange resins. 8. A systematic method for the separation of amino-acids
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چکیده
منابع مشابه
Displacement chromatography on synthetic ion-exchange resins. 1. Separation of organic bases and amino-acids using cation-exchange resins.
9. Accumulation of glutamate also occurred in isolated kidney cortex, spleen, lung and chorion. Six other tissues examined maintained a concentration gradient between tissue and medium, but did not increase the glutamate concentration above the original level. 10. It is pointed out that tissue slices afford a system in which problems of permeability and storage can be studied under controlled c...
متن کاملDisplacement Chromatography on Synthetic Ion - exchange Resins
In Part 1 of this series of papers (Partridge & Westall, 1949) it was shown that, whilst the baseexchange resin Zeo Karb 215 may be used with success for the separation of the neutral and acidic amino-acids, certain difficulties arise when the separation of basic substances is attempted. These difficulties were traced to the phenolic hydroxyl groups of the resin. Resins of the sulphonated pheno...
متن کاملDisplacement chromatography on synthetic ion-exchange resins. 2. The separation of organic acids and acidic amino-acids by the use of anion-exchange resins.
The application of the principle of displacement chromatography to the separation of bases and amino-acids on columns packed with the cationexchange resin Zeo-Karb 215 has been described in Part 1 of this communication (Partridge & Westall, 1949). It is expected that this method will prove to be of value in the isolation of bases and amino-acids from biological extracts of various kinds, and th...
متن کاملPreparative Isolation of Amino Acids by Carrier Displacement Chromatography on Ion Exchange Resins* by Donald
Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...
متن کاملPreparative isolation of amino acids by carrier displacement chromatography on ion exchange resins.
Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1952
ISSN: 0306-3283
DOI: 10.1042/bj0510628